Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene.
نویسندگان
چکیده
The endoglucanase CelB isolated from culture filtrates of Streptomyces lividans IAF9 has an M(r) of 36,000. With carboxymethyl cellulose as the substrate, the Vmax and Km values are 110 IU/mg of enzyme and 1.3 mg/ml, respectively. Comparison of primary amino acid sequences classifies CelB in the H family of cellulases.
منابع مشابه
Purification and Characterization of an Endoglucanase from Streptomyces lividans 66 and DNA Sequence of the Gene MICHELE
were a V... of 24.9 IU/mg of enzyme and a Km of 4.2 mg/ml. Activity was found against neither methylumbelliferylnor p-nitrophenyl-cellobiopyranoside nor with xylan. The DNA sequence contains one possible reading frame validated by the N terminus of the mature purified protein. However, neither ATG nor GTG starting codons were identified near the ribosome-binding site. A putative TTG codon was f...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 60 5 شماره
صفحات -
تاریخ انتشار 1994